Hygromycin B ≥80%
Lieferant: ICN Biomedicals
Hygromycin B is an aminoglycoside antibiotic produced by the bacterium Streptomyces hygroscopicus that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.
Hygromycin has been used to select drug-resistant stable transfectants after transfer of the hygromycin phosphotransferase gene in a variety of cell culture applications. It is used in gene transfer experiments as a selection agent.
Mode of action: Inhibition of protein synthesis. It interferes with translocation of the 70S ribosome by inducing the misreading of the m-RNA template in prokaryotes and eukaryotes. It selectively penetrates cells that have been rendered permeable by virus infection.
Hygromycin B is provided as a caramel-coloured solution in water. It is not prepared under sterile conditions. If a sterile solution is desired, working solutions of Hygromycin B in aqueous media can be passed through a 0.2 micron filter before use.
Freely soluble in water, methanol, buffer solution and ethanol. Practically insoluble in less polar solvents.
Bitte beachten Do not autoclave solutions of Hygromycin B.
|
Formel:
C₂₀H₃₇N₃O₁₃ MW: 527,5 g/mol |
MDL Number:
MFCD06795479 CAS-Nummer: 31282-04-9 |
Spezifikation Testergebnisse
| Synonyms | Antihelmycin; Hygromix-8 |
| Typical Working Concentration | For use as a selective agent, the suggested concentration range is 100- 800 µg/mL |
| Form | Clear Golden Yellow Liquid |
| Storage | Store at +4 °C. |
| pka | 7.1 and 8.8(Lit.) |
| Specific Activity | ~450,000 u/mL |
| Sterilization of Solutions | Solutions should be sterilized by means of a 0.2 micron filter. Do not autoclave solutions. |
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...