X-Gal (5-Brom-4-chlor-3-indolyl-ß-D-galactopyranosid)

Lieferant: G Bioscienes

Synonyms: BCIG

GENORC-213EA 1410 CHF
GENORC-213 GENORC-212
X-Gal (5-Brom-4-chlor-3-indolyl-ß-D-galactopyranosid)
X-Gal (5-Brom-4-chlor-3-indolyl-ß-D-galactopyranosid)

X-gal (5-bromo-4-chloro-3-indolyl-β-D- galactopyranoside) is used to indicate whether bacteria express the ß-galactosidase enzyme, which is encoded by the lacZ gene. X-gal is cleaved by ß-galactosidase yielding galactose and 5-bromo-4-chloro-3-hydroxyindole, which is oxidised into 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product. Thus, if X-gal and an inducer of ß-galactosidase (usually IPTG) is contained within an agar medium on a culture plate, colonies which have a functional lacZ gene can easily be distinguished by their blue colour.

Formel: C₁₄H₁₅BrClNO₆
MW: 408,63 g/mol
MDL Number: MFCD00005666
CAS-Nummer: 7240-90-6

Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Mehr About VWR