X-Gal (5-Brom-4-chlor-3-indolyl-ß-D-galactopyranosid)
Lieferant: G Bioscienes
Synonyms:
BCIG
X-gal (5-bromo-4-chloro-3-indolyl-β-D- galactopyranoside) is used to indicate whether bacteria express the ß-galactosidase enzyme, which is encoded by the lacZ gene. X-gal is cleaved by ß-galactosidase yielding galactose and 5-bromo-4-chloro-3-hydroxyindole, which is oxidised into 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product. Thus, if X-gal and an inducer of ß-galactosidase (usually IPTG) is contained within an agar medium on a culture plate, colonies which have a functional lacZ gene can easily be distinguished by their blue colour.
Formel:
C₁₄H₁₅BrClNO₆ MW: 408,63 g/mol |
MDL Number:
MFCD00005666 CAS-Nummer: 7240-90-6 |
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...