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Microplate readers, Varioskan™ LUX

Lieferant: Thermo Fisher Scientific
Microplate readers, Varioskan™ LUX
Microplate readers, Varioskan™ LUX
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Varioskan™ LUX
735-0309EA 86080 CHF
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Microplate readers, Varioskan™ LUX
Mikrotiterplatten-Photometer
Varioskan™ LUX comes equipped with a range of measurement technologies including absorbance, fluorescence intensity and FRET as standard, and with optional luminescence, AlphaScreen and time-resolved fluorescence (TRF) modules. The instrument selects the measurement wavelength using filters or monochromators, depending on which is optimal for each measurement technology.

  • Automatic dynamic range selects optimal reading range based on signal intensity
  • Smart safety controls help protect instrument and samples from user error
  • Autocalibration and self-diagnostics for confidence in results
  • Accurate temperature control for temperature critical applications
  • Integrated gas control module (optional) for setting CO₂ and O₂ concentrations for cell based assays
  • Optional dispensers to allow simultaneous dispensing and measurement for signal monitoring at the start of a reaction

SkanIt software (available in 9 languages):
Intuitive user-interface simplifies measurement setup
Virtual pipette™ tool makes it easy to define samples to plate layout
Built-in calculation options ease data processing
Manual or automatic data export to any location
Measurement data continuously saved to the database; helps prevent data loss due to interruptions such as power outage or accidental aborting
Available in 2 editions: Research Edition (included) or Drug Discovery Edition

Absorbance:
Plate types: 6 to 384 well plates
Wavelength selection: Double monochromators
Wavelength range: 200 to 1000 nm
Light source: Xenon flash lamp
Read out range: 0 to 6 Abs
Linear measurement range: 0 to 4 Abs (96-well plate) at 450 nm, ±2%; 0 to 3 Abs (384-well plate) at 450 nm, ±2%
Accuracy: 0,003 Abs or ±2%, at 200 to399 nm (0 to 2 Abs); 0,003 Abs or ±1%, at 400 to 1000 nm (0 to 3 Abs);
Precision: SD <0,001 Abs or CV <0,5%, at 450 nm (0 to 3 Abs)

Fluorescence intensity:
Plate types: 6 to 1536 well plates
Wavelength selection: Double excitation and emission monochromators
Excitation wavelength range: 200 to 1000 nm
Emission wavelength range: 270 to 840 nm
Light source: Xenon flash lamp
Sensitivity: Top reading: <0,4 fmol fluorescein/well (black 384-well plate); Bottom reading: <4 fmol fluorescein/well, (clear bottom black 384-square well plate)
Dynamic range: Top reading >6 decades; Bottom reading >5,5 decades

Time-resolved fluorescence:
Plate types: 6 to 1536 well plates
Wavelength selection: Filters (spectral scanning with double excitation and emission monochromators)
Excitation wavelength range: Fixed to 334 nm (spectral scanning 200 to 840 nm)
Emission wavelength range: 400 to 700 nm (spectral scanning 270 to 840 nm)
Light source: Xenon flash lamp
Sensitivity: <1 amol Eu/well (white low volume 384 well plate)
Dynamic range: >6 decades

Luminescence:
Plate types: 6 to 1536 well plates (spectral scanning 6 to 384 well plates)
Wavelength selection: Direct or filters (spectral scanning with double monochromators)
Wavelength range: 360 to 670 nm
Sensitivity: <7 amol ATP/well (white 384-well plate)
Dynamic range: >7 decades

AlphaScreen:
Plate types: 6 to 1536 well plates
Wavelength selection: Filters
Excitation wavelength range: Fixed to 680 nm
Emission wavelength range: 400 to 660 nm
Light source: LED
Sensitivity: <100 amol phosphotyrosine/well (white 384-well plate)

Dispensing:
Plate types: 6 to 384 well plates
No. of dispensers: None, one or two
Syringe size: 1 ml (standard), 5 ml (optional)
Dispensing volume: 2 to 5000 μl, in 1 µl increments (1 ml syringe); 5 to 25 000 μl, in 5 µl increments (5 ml syringe)
Accuracy: <1 μl with 50 μl (0,4 mm tip), <0,2 μl with 5 μl (0,25 mm tip)
Precision: <1 μl with 50 μl (0,4 mm tip), <0,25 μl with 5 μl (0,25 mm tip)
Dead volume: Reagent loss <100 μl; Total tubing volume <800 μl

Bestellinformation: For further technical specifications relating to all the available modules for the Varioskan® LUX and onboard dispenser(s), incubator and shaker please contact your local Avantor sales office.
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