The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.
- Chemically defined - consistent product performance, minimal lot-to-lot variability
- Autoinduction media simplifies protocol by eliminating the monitoring and induction steps
- Sterile stock solutions minimise sample handling, significantly reduce the chance of contamination and convenient
With Overnight Express™ Autoinduction Systems, a period of cell growth is followed by spontaneous induction of protein expression – without monitoring cell density and without conventional induction with IPTG. The method is based on media components that are metabolised differentially to promote growth to high density and automatically induce protein expression from lac promoters.
The Overnight Express™ Autoinduction System is extremely convenient for routine expression of proteins in multiple cultures and is ideal for high-throughput parallel analysis of protein expression, solubility, and purification from multiple expression clones.
Overnight Express™ Autoinduction System 2: Features the capability to label proteins with selenomethionine (Se-Met) for downstream crystallisation and x-ray diffraction studies. All components is chemically defined, resulting in consistent product performance by minimising lot-to-lot variability.
The Overnight Express™ Autoinduction System 2 kit contains six concentrated sterile solutions:
OnEx™ Solution 1: A blend of carbon sources optimised for tightly regulated uninduced growth to high cell density, followed by high-level induction
OnEx™ Solution 2: A concentrated buffer and nitrogen-source bend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis
OnEx™ Solution 3: Provides magnesium for maximal cell density
OnEx™ Solution 4: Provides trace metals to minimise growth limitations associated with mineral deficiencies and satisfies the metal requirements of metal-containing target proteins, even at high expression levels
OnEx™ Solution 5: A mixture of amino acids lacking methionine
OnEx™ Solution 6: A separate methionine solution. Sufficient methionine (Met) is provided to support growth of the Met auxotroph B834 while providing the ability to reduct the level of unlabelled Met for slenomethionine incorporation by Met auxotrophs
The Overnight Express Autoinduction System 2 is the perfect solution for routine expression of proteins in multiple cultures and is ideal for high-throughput parallel analysis of protein expression, solubility screening, and purification from multiple expression clones.
Lieferumfang: Each 1 L kit includes OnEx™ Solution 1: 1×20 ml, OnEx™ Solution 2: 1×50 ml, OnEx™ Solution 3: 1×1 ml, OnEx™ Solution 4: 1×1 ml, OnEx™ Solution 5: 1×20 ml, OxEx™ Solution 6: 1×20 ml. Each 5 L kit includes OnEx™ Solution 1: 5×20 ml, OnEx™ Solution 2: 2×125 ml, OnEx™ Solution 3: 5×1 ml, OnEx™ Solution 4: 5×1 ml, OnEx™ Solution 5: 1×100 ml, OnEx™ Solution 6: 1×100 ml.
