E.Z.N.A.® Plant & Fungal DNA Kit
Lieferant: OMEGA BIO-TEK
Isolate DNA from plant or fungal samples using spin columns.
- Rapid – DNA isolation under 60 minutes
- Reliable – Optimized buffer system ensure the reproducible results
- Safe – No organic extractions
- High-quality – Purified DNA suitable for most downstream applications
The E.Z.N.A.® Plant & Fungal DNA Kit allows for the rapid and reliable isolation of high-quality total cellular DNA from a wide variety of plant or fungal species and tissues. Up to 200 mg wet tissue (or 50 mg dry tissue) can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of Omega Bio-tek’s HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant and fungal tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.
If you are using the E.Z.N.A.® Plant and Fungal DNA Kit for the first time, please read this booklet to familiarize yourself with the procedure and its various modifications. Plant or fungal tissue, either dried or fresh, is disrupted and lysed in a specially formulated buffer containing detergent. Proteins, polysaccharides, and cellular debris are subsequently precipitated. Contaminants are further removed by an isopropanol precipitation step. Binding conditions are adjusted, and the sample is transferred to a HiBind® DNA mini column. Two rapid wash steps remove residual contaminants, such as residual polysaccharides, and pure DNA is eluted in water or a low ionic strength buffer.
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