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Sie suchten nach: Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.


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Product Image-PIER34065

DAB substrate kit, metal enhanced, Pierce™

Artikel-Nr: (PIER34065)
Lieferant: Thermo Fisher Scientific
Hersteller Artikel Nummer : 34065
Beschreibung: The Pierce™ Metal enhanced DAB substrate kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense colour for immunohistochemical staining.
UOM: 1 * 250 mL
Product Image-786-123

Sample clean up, PAGE-Perfect™

Lieferant: G-Biosciences
Beschreibung: Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or interfering agents, such as salts, acids, bases and detergents, result in band distortion and poor protein resolution. As a result, SDS-PAGE gels are hard to analyse and lack reproducibility. PAGE-Perfect™ is a simple, two-step method for concentrating, cleaning and preparing protein solutions for running publication quality gels.

Image Unavailable-69078-3

Western blotting kit, Perfect Protein™, Novagen®

Artikel-Nr: (69078-3)
Lieferant: Merck Millipore (Novagen)
Hersteller Artikel Nummer : 69078-3
Beschreibung: The Perfect Protein™ HRP Western Blot Kit includes an HRP or AP conjugate in addition to Perfect Protein™ Western markers.
UOM: 1 * 1 ST
Product Image-ROCKFEMTOMAX-020

Chemiluminescent super sensitive HRP substrate, FemtoMax™

Lieferant: Rockland Immunochemicals
Beschreibung: FemtoMax™ is an extremely sensitive, non radioactive, and enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). It allows for the detection of femtogram (10 to 15) amounts of antigen using photographic film or other imaging methods including highly sensitive CCD cameras.

Image Unavailable-J60561.AP

SSC (20X), RNAse free

Lieferant: Thermo Fisher Scientific
Beschreibung: SSC solution is commonly used in nucleic acid hybridization techniques at concentrations ranging from 0,1X to 20X depending on the application. It is used to facilitate transfer of nucleic acids to membranes in Southern and Northern blotting, dot blotting, and colony and plaque lifts.

Sicherheitsdatenblätter Zertifikate

Product Image-PIER35065

Fast western blotting kit, SuperSignal™ west Pico, Pierce™

Lieferant: Thermo Fisher Scientific
Beschreibung: The Fast Western Blot Kit, SuperSignal™ West Pico Substrate contains optimised reagents and protocol for picogram-level detection and and Western blot development less than 1 hour after gel transfer.

Product Image-ROCK18-8817-31

Western blot kits, TrueBlot®

Lieferant: Rockland Immunochemicals
Beschreibung: TrueBlot® Western blot kits contain the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot® second step immunoblotting reagents in conjunction with the user's own primary IP antibody and primary (mouse, goat or rabbit IgG) Western blotting antibody.

Sicherheitsdatenblätter

Image Unavailable-WB59-100

Western-Re-Probe™ reagent, Calbiochem®

Artikel-Nr: (WB59-100)
Lieferant: Merck Millipore (Oncogene)
Hersteller Artikel Nummer : WB59-100
Beschreibung: This Western-Re-Probe™ reagent is validated for use in immunoblotting.
UOM: 1 * 100 mL
Product Image-PIER35081

SuperSignal™, Pierce™ Fast Western Blotting Kit, West Femto

Lieferant: Thermo Fisher Scientific
Beschreibung: The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.

Image Unavailable-J63209.AK

Saponin permeating solution, 0,5% w/v solution in PBS (10X)

Artikel-Nr: (J63209.AK)
Lieferant: Thermo Fisher Scientific
Hersteller Artikel Nummer : J63209.AK
Beschreibung: Saponin permeating solution, is used as a cell permeabilising agent.
UOM: 1 * 250 mL

Sicherheitsdatenblätter Zertifikate


Product Image-786-124

Sample clean up, Perfect-FOCUS™

Lieferant: G-Biosciences
Beschreibung: Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.

Image Unavailable-J61462.AK

Luminol and oxidising solutions

Lieferant: Thermo Fisher Scientific
Beschreibung: For chemiluminecent HRP-based detection of western blotting systems. It is soluble in most polar organic solvents but is insoluble in water.

Sicherheitsdatenblätter Zertifikate

Image Unavailable-A3417.5000

CheLuminate-HRP PicoDetect Chemilumineszenz-Nachweiskit

Artikel-Nr: (A3417.5000)
Lieferant: PanReac AppliChem
Hersteller Artikel Nummer : A3417.5000
Beschreibung: Das CheLuminate-HRP PicoDetect ist ein komplettes Kit mit gebrauchsfertigen Reagenzien für die Chemilumineszenz-Nachweis von immobilisierten Proteinen (Western Blot) oder immobilisierten Nukleinsäuren (Southern oder Northern Blot), die direkt oder indirekt mit Meerrettichperoxidase (HRP) konjugiert sind.
UOM: 1 * 1 KIT
Product Image-ROCKKCA004

Chemiluminescent western blot kits, FemtoMax™

Lieferant: Rockland Immunochemicals
Beschreibung: These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECL™ substrate is the equivalent of a 1:5000 dilution using the FemtoMax™ substrate.

Product Image-PIER35071

Fast western blot kit, SuperSignal™ West Dura, Pierce™

Lieferant: Thermo Fisher Scientific
Beschreibung: The Fast Western Blot Kits, SuperSignal™ West Dura provide reagents and protocol for reliable and rapid, 1 hour chemiluminescent Western blotting at femtogram levels of sensitivity. These complete Fast Western Blot Kits requires minimal hands on time and yield results comparable to classical Western blotting with SuperSignal™ West Dura Chemiluminescent Substrate. This substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse or rabbit primary antibody.

Product Image-TB110

Kingdom blot

Artikel-Nr: (TB110)
Lieferant: G-Biosciences
Hersteller Artikel Nummer : TB110
Beschreibung: Kingdom blot is prepared using total protein extracted from a variety of species, where whole species is not available the whole liver lysate is used. These blots can be used to identify similar proteins in other species, including human, chicken, frog, worm, Drosophila, yeast, Aspergillus, <i>E. coli</i>, Rhizobium and green algae.
UOM: 1 * 1 ST
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