Sie suchten nach: RT-PCR und cDNA Reagenzien

Lieferant: Thermo Fisher Scientific

Beschreibung: RevertAid™ H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid™ H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

Artikel-Nr: (71978-3)

Lieferant: Merck Millipore (Novagen)

Hersteller Artikel Nummer : 71978-3

Beschreibung: The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots,<i> in situ</i> hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant <i>Thermus thermophilus</i> (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.

UOM: 1 * 1 KIT

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Artikel-Nr: (27-9261-01)

Lieferant: Cytiva

Hersteller Artikel Nummer : 27-9261-01

Beschreibung: Designed to generate full length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for <i>in vitro</i> amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method.

UOM: 1 * 55 Tests

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Artikel-Nr: (27-9264-01)

Lieferant: Cytiva

Hersteller Artikel Nummer : 27-9264-01

Beschreibung: Ready-To-Go™ You-Prime First-Strand beads are preformulated, single-dose reaction beads prepackaged in thin-walled 0,5 ml tubes compatible with most thermal cyclers. Ready-To-Go™ You-Prime First-Strand beads are used for synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice.

UOM: 1 * 50 Tests

Sicherheitsdatenblätter Sale

Lieferant: Cytiva

Beschreibung: Vordosierte, gefriergetrocknete Beads, die die Reagenzien enthalten, die für die einstufige reverse Transkriptions-PCR mit hoher Empfindlichkeit und Reproduzierbarkeit erforderlich sind.