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Lieferant: G-Biosciences

Beschreibung: Antibody Fab Fragmentation Kits are designed for the generation and isolation of Fab fragments from IgG molecules. The kits utilise Immobilised Papain resin. Immobilised Papain offers the advantage of generating Fab and fragments without the need to remove the papain enzyme after digestion. Following papain digestion the Fab fragments are separated from undigested IgG and the Fc region with the supplied Protein A Spin Column. Protein A Resin binds the IgG and Fc molecules and the Fab are rapidly collected.

Lieferant: G-Biosciences

Beschreibung: The F(ab)₂ Fragmentation kits are designed for the generation and isolation of F(ab)₂ fragments from IgG molecules. The kit utilises Immobilised Pepsin resin. Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)₂ fragments from immunoglobulin G (IgG). One or more of the disulfide bonds that join the heavy chains in the hinge region are preserved, so the two Fab regions of the antibody remain joined together, yielding a divalent molecule (containing two antibody binding sites), hence the designation F(ab)₂. The light chains remain intact and attached to the heavy chain, whereas the Fc fragment is digested into small peptides. The Immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.

Lieferant: Thermo Fisher Scientific

Beschreibung: B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.

Artikel-Nr: (786-129)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : 786-129

Beschreibung: A reagent kit for detection and estimation of SDS in a sample. Mix the test sample in the extraction buffer reagents provided with the kit. If SDS is present in the sample, a blue colour is extracted that can be quantitatively measured.

UOM: 1 * 1 KIT

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Artikel-Nr: (PIER88279)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : 88279

Beschreibung: The Pierce™ Mouse Embryonic Fibroblast (MEF) Isolation Kit enables high yield isolation and culture of viable primary fibroblasts from mouse embryos.

UOM: 1 * 1 KIT

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Artikel-Nr: (786-018)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : 786-018

Beschreibung: FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins.

UOM: 1 * 1 KIT

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Artikel-Nr: (786-246)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : 786-246

Beschreibung: Extracts and solubilises nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilise even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.

UOM: 1 * 1 KIT

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Artikel-Nr: (786-249)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : 786-249

Beschreibung: FOCUS™ Membrane Proteins for the fractionation of highly enriched membrane proteins.

UOM: 1 * 1 KIT

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Artikel-Nr: (786-257)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : 786-257

Beschreibung: Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilises nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, which has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.

UOM: 1 * 1 KIT

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Lieferant: G-Biosciences

Beschreibung: Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG₁ Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.

Lieferant: Thermo Fisher Scientific

Beschreibung: B-PER® Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. The B-PER® Bacterial Protein Extraction Kit eliminates the need for harsh mechanical procedures, like sonication. The easy-to-use cell lysis solution uses mild non-ionic detergents to disrupt cells and solubilise proteins without denaturation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER® is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilised metal affinity chromatography (IMAC).

Artikel-Nr: (786-182)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : 786-182

Beschreibung: The Nuclear and Cytoplasmic Extraction kit is for the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues. The kit generates a cleaner separation of cytoplasmic proteins from nuclear proteins and is ideal for expression and transport studies.

UOM: 1 * 1 KIT

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Artikel-Nr: (786-231)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : 786-231

Beschreibung: The FOCUS™ Reduction-Alkylation Kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis.

UOM: 1 * 1 KIT

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Artikel-Nr: (786-230)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : 786-230

Beschreibung: FOCUS™ Protein Reductant Kit is a water soluble, odorless, non-toxic and stable TCEP [Tris (2-carboxyethyl) phosphine] reductant for protein reduction.

UOM: 1 * 1 KIT

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Artikel-Nr: (PIER89899)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : 89899

Beschreibung: The Pierce™ Ubiquitin enrichment kit facilitates the isolation of polyubiquitin protein conjugates from cultured cells and tissue samples.

UOM: 1 * 1 KIT

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Artikel-Nr: (PIER84868)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : 84868

Beschreibung: Das Pierce™ Umkehrphasen-Kit mit hohem pH-Wert erhöht die Proteinidentifizierung aus LC/MS-Analysen durch orthogonale Peptidfraktionierung komplexer Peptidproben.

UOM: 1 * 1 KIT

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